Biotechnology Lab Report<\/p>\n
Task:<\/p>\n
Describe in Scientific Paper Format (SPF) the experiments you performed for practical 3 (the last one involving PCR
\nBS934 Assignment 3
\nPlease complete the assignment by yourself. We screen for plagiarism and collusion.
\nThe assignment should be not more than 4 A4 pages, maximal word counts of each section as outlines below, font size 11 Ariel or Helvetica, single line spacing, without counting figure legends and references. Anything that is beyond these limits will be ignored.
\nDeadline for return of the assignment is 6th December 2022, 16:00.
\n————————————————————————————————————–Task:
\nDescribe in Scientific Paper Format (SPF) the experiments that you performed for practical 3 (the last one involving PCR).
\nThe assignment should have following elements:
\n1.\tTitle (max 10 words)
\n2.\tAbstract: not more than 75 words (summarise what was done).
\n3.\tIntroduction: Summarise the background and aims, 200 words max. You can extract this from the practical handbook. This should be very succinct, you can refer to the Scribble gene and what it does briefly and reference the relevant paper cited in the practical handbook. You can refer to CRISPR-Cas9 technology, but only briefly and again cite relevant literature.
\n4.\tMethods section (700 words max): describe in brief terms what has been done, like in a scientific paper. Everything should be written in past tense. Look at published papers how such methods are written. For example, look at what was in the practical handout:
\n\u201c DNA will be amplified using the following programme:<\/p>\n
94 \u00b0C 5 min
\n94 \u00b0C 30 s
\n52 \u00b0C 30 s\t\tx 40 cycles
\n72 \u00b0C 30 s
\n72 \u00b0C 7 min\u201d
\nShould become:
\n\u201cPCR was performed with following parameters: initial denaturation: 94 \u00b0C 5 min, then 40 cycles of 94 \u00b0C 30 s, 52 \u00b0C 30 s, 72 \u00b0C 30 s followed by 72 \u00b0C, 7 min.\u201d<\/p>\n
Or:
\n\u201cCarefully pipette off and discard as much liquid as you can, leaving the cell pellet\u201d<\/p>\n
Should become: \u201csupernatant was removed\u201d. And
\n\u201cUsing a P1000 carefully add 250 \u00b5l of your sample to the 1.5 ml microcentrifuge tube in the rack. This tube already contains 10 \u00b5l of PT-L2P DNA extraction buffer.\u201d
\nBecomes:
\n\u201c250 \u00b5l of the DNA sample was added to a tube containing 10 \u00b5l of PT-L2P DNA extraction buffer\u201d.<\/p>\n
This:
\nAdd the following reagents to both tubes, in the order specified:
\n10 \u00b5l \tPCR product
\n7 \u00b5l \tsterile H2O \t\t\t(OR ADD 8 \u00b5l to tube N)
\n2 \u00b5l \t10x digestion buffer
\n1 \u00b5l \tHaeIII restriction enzyme\t(DO NOT ADD enzyme to tube N)
\nNote: take your reaction mix and a pipette to a demonstrator who has the restriction enzyme stock on ice.
\nShould become:
\n\u201cThe DNA was digested using HaeIII restriction enzyme\u201d.
\nThis: \u201cIncubate at 37\u00baC in a water bath for 1 hr\u201d becomes: the sample was incubated 1 h, 37\u00baC.
\nSteps you did not do (preparation of bacterial plates) you do not need to describe.
\nYou can use tables to summarise some protocol steps, but only use maximally 2.
\nYou can also refer to manufacturer\u2019s protocols (e.g. GeneJet) and not describe these in detail.
\n5.\tResults: 200 words max. Describe briefly what was done (summarise the methods steps in a few sentences) and what is seen on the gels, refer to the results figures (e.g., \u201c..as shown in Figure 1..\u201d.
\n6.\tResult Figures:
\nGenerate result figures (Figures 1 and 2 of the two gels you ran) with legend from your results generated (uploaded on Moodle). Legend should be brief but clear, so it is obvious what is shown on the gel. You can include a figure that shows what was expected and compare the results in the Discussion. If you have images of the bacterial plates (blue\/white screening) you can show that, but this is not necessary.<\/p>\n
FIGURE PRESENTATION
\nCut and paste\/insert your gel images from Moodle into POWERPOINT, where you can label the gel. Save the labelled image as a jpeg or pdf to insert into your worksheet, label markers on your own gels (we used a 100 bp ladder).
\nLabelling gels: avoid any writing over the gel; do not try to label every size marker band; informative labelling makes the figure easier to understand than just numbering the tracks. (however, you can just number the lanes and then refer to the label numbers in the legend).
\nFigure legend must be below the figure and:
\n\u2022\tmust contain a number and brief title for the figure;
\n\u2022\tshould name the method (s, e.g. DNA separation by agarose gel electrophoresis) and have sufficient detail to enable the content of the figure to be understood without reading the text;
\n\u2022\tshould include a key to explain labelling if necessary;
\n\u2022\tmust NOT report, interpret or explain the results.
\nIf you gel did not show the expected results (e.g., no band), still show it, as this will give you the opportunity to discuss what might have gone wrong in DISCUSSION. But you can also show the results from another student that gave expected results and compare. Or use someone else\u2019s results, if you did not get any (if for some reason, your samples were not run, us results from someone else). If you combine images of two different gels or sections of gels, clearly indicate the two sections (do not splice gels together so it looks like it is one gel, this is image manipulation).
\n7.\tDiscussion: 200 words max. How do the results compare with what was expected? Why might the experiment not have brought you the expected results, if this is the case?
\n8.\tReferences: Any references you deem necessary, including any protocols from manufacturers (e.g. GeneJet).
\n9.\tAssessment Criteria. These elements are not necessarily given equal weight in the overall assessment. (Marker: please highlight relevant comments.)
\nElements \tTop distinction
\n>80% \tDistinction 70%-79% \tMerit 60%-69% \tPass 50%-59% \tFail <50% \tLow fail <40%
\nCompleteness \tAll sections completed. \tAll sections completed. \tAlmost all sections completed. \tMost sections completed \tSome sections not completed. \tMany \/ most sections not completed.
\nQuality of answers. \tAll the info. required.
\nAll accurate, relevant and concise. \tAll important material, concise, few minor errors. \tConsiderable content.
\nMinor errors, some irrelevant material. \tReasonable info. content. Minor and some major errors. Some irrelevant material. \tLimited answers, and\/or many major errors, not concise, some waffle. \tLittle or no valid info.
\nMany errors, most material irrelevant and\/or waffle.
\nResults.
\n(as appropriate) \tVery clearly, selectively & concisely described. Data correctly analysed & interpreted.
\nTables and figures neat, accurate &, fully labeled.
\nStatistics correctly used, interpreted, & reported. \tClearly & concisely described. Data correctly analysed & interpreted. Tables and figures neat, accurate & fully labeled.
\nStatistics correctly used, interpreted & reported. \tMostly clearly & concisely described. Data generally well analysed; few misinterpretations.
\nSome omissions \/ errors in presentation of tables and figures.
\nStatistics mainly correctly used, interpreted, & reported. \tGenerally clear, but difficult to identify key results.
\nData analysis & interpretation satisfactory but some errors \/ omissions.
\nSignificant errors in presentation of tables & figures.
\nSome errors in use, reporting & interpretation of statistics. \tUnclear, key results not identified. Many errors \/ omissions in data analysis & interpretation.
\nMany errors in presentation of tables & figures.
\nMajor errors in use, reporting & interpretation
\nof statistics. \tNo description of results.
\nData not analysed; all key points missed or misinterpreted.
\nTables and figures very poor or missing.
\nStatistics not used or totally inappropriate.
\nUnderstanding shown \tExcellent. \tClearly evidenced. \tSubstantial evidence. \tSome evidence. \tLimited and patchy. \tLittle or none.<\/p>\n
Presentation\/
\nQuality of writing. \tGreat clarity. Very concise. Entirely logical in structure. Negligible errors in spelling & grammar. \tClear and concise. Generally very logical. Minimal errors in spelling & grammar. \tUsually clear and concise.
\nOnly minor weaknesses in logic.
\nFew minor errors in spelling & grammar. \tSome lack of clarity and not concise.
\nSome lapses in logic.
\nSome errors in spelling & grammar. \tLacks clarity, and not concise. Frequently illogical.
\nMany errors in spelling & grammar. \tRambling, unclear. Difficult to understand. Illogical.
\nVery many errors in spelling & grammar.<\/p>\n","protected":false},"excerpt":{"rendered":"
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